On-Campus Facilities and Experts
Ithaca
Flow Cytometry instrumentation is available in a main facility in 140 Biotechnology Building and a satellite facility in VRT T2-003 of the College of Veterinary Medicine. The facility houses four cell sorters and three analyzers. Read more at the CIB Flow Cytometry Core Facility Website
Contact: Lydia Tesfa
Weill Cornell
The WCM CLC Flow Cytometry Core Facility offers state-of-the-art fluorescence activated cell sorting (FACS) instruments and services, and expertise in their applications, to the Weill Cornell Medicine (WCM) community and to outside investigators. The facility also provides consultation on project design and data analysis, and offers educational workshops, seminars and training. Read more at the WCM CLC Flow Cytometry Core Facility Website
Contact: Jason McCormick or Tom Baumgartner – 646-962-7611
Panel Design
Ithaca Specific
• Fluorofinder Panel Design
• Panel Design Dos & Don’ts – L. Tesfa
Other
• Astrolabe Antibody Staining Data Set
• BD Guided Panel Solution
• BD Spectrum Viewer
• BenchSci Ab Search
• Bio-Rad Antigen Density
• Chromocyte Panel Builder
• Fluorofinder
• Thermo Spectra Viewer
• Thermo Panel Builder
• UC Flow Panel Design Overview
Data
Analysis
The Flow Cytometry Core Facility has partnered with Weill Cornell Medicine and the BRC Bioinformatics facility for access to FlowJo and FCS Express software. If you are interested in acquiring a license for FlowJo or FCS Express, please contact bio-it@cornell.edu. Please include your name, your PI’s name and a valid Cornell account number. (http://www.biotech.cornell.edu/node/137). FlowJo software is also available on the Flow Cytometry Facility Shared workstation for any user of the facility subject to schedule availability and access to the lab.
Repositories
• FlowRepository
• ImmPort
• ImmuneSpace
• Minimum Set of Research Flow Cytometry Standards (MiSET RFC)
• Minimum Information about a Flow Cytometry Experiment (MIFlowCyt)
Textbooks
• Practical Flow Cytometry – Howard Shapiro
📘 Physical copy | Digital copy
• Flow Cytometry: First Principles – Longobardi
Digital copy
• Imaging Flow Cytometry: Methods and Protocols – Barteneva & Vorobjev
Digital copy
• Flow Cytometry: Principles and Applications – Macey
📘 Physical copy | Digital copy
Virtual Resources
Communities
• Chromocyte
• International Society for Advancement of Cytometry
• Scientific Community Imaging Forum
• Purdue University Cytometry Laboratories
Tips, Tutorials, and Protocols
• BD Biosciences – Flow Cytometry Web-Based Training
• BioLegend for Spectral Cytometry
• Bio-Rad – Introduction to Flow Cytometry
• Boster Bio – Flow Technical Resource Center
• CCEN e-learning
• Compensation in Flow Cytometry Analyses – Roederer
• Current Protocols in Cytometery and Immunology – Wiley
• Cytek Full Spectrum Viewer
• CYTO U – International Society for Advancement of Cytometry
• Expert Cytometry
• Flowbook – FCS Express
• FlowJo University
• FlowJo Webinars
• Protocols for Fluorescence Conjugation of Antibodies – Roederer
In-person Training Opportunities
• Annual Course in Flow Cytometry
Offered by the University of Wisconsin, Madison Campus
• Comprehensive Course in Flow Cytometry
Offered by the University of California, Davis Campus
• Annual Practical Course in Clinical Cytometry
Offered by the Clinical Cytometry Education Network
Vendor Websites
